The purpose of this study was to evaluate for the cytotoxicity of root canal sealers-Tubliseal, N2, AH26, and Sealapex - on fibroblasts cultivated from human periodontal ligament. Succinate dehydrogenase activity test and 51Cr release test were
performed to evaluate the shortterm cytotoxicity. According to 1,3 and 6 days vital cell count and 14C-leucine incorporation rate to fibroblasts were evaluated.
@ES The results of this study were as follows:
@EN 1. In the test of SDH activity by millipore filter method, Sealapex showed mild cytotoxicity but Tubliseal, N2 and AH26 showed severe cytotoxicity.
2. In 51Cr release test, Tubliseal was the most cytotoxic sealer tested, and rank ordered the relative cytotoxicity of the other sealers tested as follows: AH26, N2, Sealapex.
3. In the test of viable cell count, cytotoxicity in Tubliseal was continued because vital cell number reduced with time. Because vital cell was not showed in N2 and AH 26 at 1 day, it was recognized that N2 and AH26 exhibited sever
cytotoxicity.
In
Sealapex, vital cell number increased remarkably with time, so it showed that cytotoxicity decreased with time.
4. In 14C-leucine incorporation rate test, protein sythesis was not produced in Tubliseal, N2 and AH26 after 3 days, it showed that cytotoxicity in Tubliseal, N2 and AH 26 was severe. Although protein synthesis in Sealapex decreased with time,
it
continued after 6 days. Therefore Sealapex has been exhibited mild cytotoxicity.
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